Extract from Periostracum cicadae Inhibits Oxidative Stress and Inflammation Induced by Ultraviolet B Irradiation on HaCaT Keratinocytes

Figure 1

P. cicadae reduced the ROS production of HaCaT cells after UVB exposure. HaCaT cells were treated with 20 μg/mL of P. cicadae for 4 hours, followed by exposure to 12 mJ/cm² of UVB. The levels of ROS were measured by the DCFH-DA assay. (a) Fluorescence intensity of untreated control cells was set as 1 and results were expressed in X-fold. Trolox was used as an antioxidant control. H₂O₂ were used to generate hydroxyl radicals. P. cicadae effectively prevented UVB-produced ROS in HaCaT cells. (b) Representative immunofluorescent microscopy images, 40x magnification; scale  μm. was considered as a significant difference compared with the only UVB-irradiated group. was considered as a highly significant difference compared with the UVB-irradiated only group.

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