In Vivo Subacute Toxicity and Antidiabetic Effect of Aqueous Extract of Nigella sativa

Figure 2

Effect of AqE.NS on morphological features of mice’s liver and kidney observed using optic microscopy. ((a) G ×10, (a1) G ×40): control. ((b) G ×10, (b1) G ×40): 2 g/kg. ((c) G ×10, (c1) and (c2) G ×40): 6.4 g/kg. ((d) G ×10, (d1) and (d2) G ×40): 21 g/kg. ((e) G ×10, (e1) and (e2) G ×40): 33 g/kg. ((f) G ×10, (f1) G ×40): 60 g/kg. CV: centrilobular vein; HA: hepatic artery; BD: bile duct; MB: Mallory body; blue arrow: necrotic zone; black arrow: dilatation of sinusoids; red arrow: richness of vascularization; green arrow: fibrosis zone; yellow arrow: inflammatory zone; red arrowhead: hemorrhagic zone; black arrowhead: round cells with large nuclei. ((g) Gr ×10, (g1) Gr ×40): control. ((h) G ×10, (h1) G ×40): 2 g/kg. ((i) G ×10, (i1) G ×40): 6.4 g/kg. ((j) G ×10, (j1) and (j2): G ×40): 21 g/kg. ((k) G ×10, (k1), (k2), and (k3) G ×40): 33 g/Kg. ((l) G ×10, (l1) and (l2) G ×40): 60 g/Kg. Black arrow: glomeruli; red arrow: collecting tubules; blue arrow: glomeruli removed from their vascular pellet; green arrow: distal convoluted tubule; yellow arrow: proximal convoluted tubule; black arrowhead: expanded Bowman space; red arrowhead: blood vessel. M: medulla; C: cortex.