Antineuroinflammatory Effects of Modified Wu-Zi-Yan-Zong Prescription in β-Amyloid-Stimulated BV2 Microglia via the NF-κB and ERK/p38 MAPK Signaling Pathways

Figure 2

MWP inhibits the mRNA expression of inflammatory mediators, as well as the protein expression of iNOS in Aβ-activated BV2 microglia. BV2 microglia were incubated with Aβ (20 μM) in the presence or absence of MWP (50, 100, and 200 mg/L) for 6 h. mRNA expressions of IL-6, TNF-α, IL-1β, and MCP-1 were tested (a–d). BV2 microglia were incubated with Aβ (20 μM) in the presence or absence of MWP for 24 h. Total protein of the cells was extracted and used to detect the expression of iNOS by western blot (e). All values are expressed as mean ± standard deviation of triplicate tests. and relative to the control group; and relative to the Aβ group (see Table 3).

(a)

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