Anticancer Efficacy of Cordyceps militaris Ethanol Extract in a Xenografted Leukemia Model

Figure 2

Effect of Cm-EE on apoptotic cell death and survival pathways. (a) RMA cells (2 × 10⁶ cells/ml) were incubated with Cm-EE for 24 h. Viability of RMA cells was then evaluated by MTT assays. (b) Cell apoptosis was determined by Annexin V/7ADD staining. Annexin V⁺ (right lower quadrant) and Annexin V⁺/7AAD⁺ (right upper quadrant) cells indicate early apoptotic and late apoptotic cell levels. The levels of Cm-EE-treated apoptotic cells were evaluated by flow cytometry. (c) Levels of phosphorylated Akt, p85, and GSK3β in Cm-EE-treated RMA cells were detected by immunoblotting assays.

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