Protective Effect of Tang Wang One Decoction on the Retinal Vessels of Diabetic Rats

Figure 4

Effects of TWOD on the number of endothelial cells and pericytes. TWOD group and CaD group received a single dose of 60 mg/kg STZ to induce diabetes and control rats received vehicle. TWOD group was treated with 10 g/kg TWOD. CaD group was treated with 250 mg/kg CaD. Control group and model group were treated with the same volume of vehicle. The retinas were prepared with trypsin digestion and stained with periodic acid-Schiff stain. It was observed that endothelial cells proliferated (a). Parts of the pericytes underwent apoptosis and were replaced with ghost pericytes (b). Endothelial cells and pericytes were counted under a microscope (c). Results were analyzed by one-way ANOVA, followed by a LSD post hoc test for multiple comparisons. Values are demonstrated as mean ± SD; ; versus control group; versus model group.

(a)

(b)

(c)