Evidence-Based Complementary and Alternative Medicine

Research Article

Chemical Differentiation of Dendrobium officinale and Dendrobium devonianum by Using HPLC Fingerprints, HPLC-ESI-MS, and HPTLC Analyses

Table 1

Characterization of compounds from the extract of D. officinale stems by HPLC-ESI-MS (in negative mode). A total of 26 compounds were identified, including 14 flavonoids, 5 phenolic acids, 1 lignan, and 6 other compounds from the stems of D. officinale.


Compound		m/z observed	MS² m/z observed	Formula	Identification

1	3.20	165.07	147.02(100), 129.00(60), 118.87(27)		To be identified
2	3.25	167.04	149.08(100), 123.29(10), 85.88(13), 75.09(80)		To be identified
3	3.67	192.96	177.09(43), 148.93(51)	C₁₀H₁₀O₄	Ferulic acid
4	16.84	286.91	268.91(20), 241.00(100), 135.11(10)		To be identified
5	18.86	329.01	285.16, 165.08(100), 121	C₁₉H₂₂O₅	Dihydroconiferyldihydro-p-coumarate
6	18.74	167.05	152.03(30), 123.07(100), 108.11(5)	C₈H₈O₄	Vanillic acid
7	20.66	197.05	182.07(100), 153.09(50), 137.86	C₉H₁₀O₃	Syringic acid
8	24.30	293.03	131.07(100)		To be identified
9	30.00	593.48	533.22(8), 503.15(22), 473.13(66), 353.17(6)	C₂₇H₃₀O₁₅	Vicenin-2
10	32.05	563.48	503.17, 473.10(100), 443.12(60), 353.14(13)	C₂₆H₂₈O₁₄	Apigenin-6-C-β-D-xyloside-8-C-β-D-glucoside
11	33.68	612.84	553(40), 539.16(100), 492.96(40), 459.01(30), 451.06(50), 293.28(10)		To be identified
12	33.80	563.18	503.20(60), 473.10(100), 443.09(64), 353.20(8)	C₂₆H₂₈O₁₄	Isoschaftoside
13	35.93	563.58	503.20(22), 473.17(47), 443.16(41), 353.14(7)	C₂₆H₂₈O₁₄	Schaftoside
14	36.57	593.67	473.10(10), 413.12(100), 293.17(6)	C₂₇H₃₀O₁₅	Vitexin-2′′-O-β-D-glucopyranoside
15	36.61	163.08	163.16, 119.29	C₉H₈O₃	p-Hydroxycinnamic acid
16	37.12	563.21	503(4), 473.17(22), 443.13(20), 353.03(6)	C₂₆H₂₈O₁₄	Apigenin-6-C-β-D-glucoside-8-C-β-D-xyloside
17	38.04	533.58	515.15(30), 473.24(40), 443.15(100), 383.08(10)	C₂₅H₂₆O₁₃	Apigenin-6-C-β-D-xyloside-8-C-β-D-arabinoside
18	38.38	533.17	515.18(22), 473.18(40), 443.16(80)	C₂₅H₂₆O₁₃	Apigenin-6,8-di-C-α-L-arabinoside
19	38.61	577.07	559.2(28), 503.02(20), 487.20(50), 473(30), 457.15(100), 383.20(23)	C₂₇H₃₀O₁₄	Violanthin
20	38.82	577.22	559.2(42), 503.19(78), 473.15(76), 457.13(100), 383.15(40)	C₂₇H₃₀O₁₄	Isoviolanthin
21	39.37	596.24	434.01(100)		To be identified
22	39.62	609.04	301.05(100)	C₂₇H₃₀O₁₆	Rutin
23	41.22	533.55	515.15(26), 473.16(46), 443.15(72)	C₂₅H₂₆O₁₃	Apigenin-6-C-α-L-arabinoside-8-C-β-D-xyloside
24	44.03	563.10	473.13(15), 383.16(100)	C₂₆H₂₈O₁₄	Apigenin-6-C-(2′′-O-β-D-glucopyranoside)-α-L-arabinoside
25	45.02	417.02	402.12(40), 387.16(12), 181.12(100), 166.09(60)	C₂₂H₂₆O₈	Syringaresinol
26	66.21	271.23	177.15(15), 151.08(80)	C₁₅H₁₂O₅	Naringenin

: retention time. : to be identified.