PC2 Ovotransferrin: Characterization and Alternative Immunotherapeutic Activity

Figure 3

AGID testing of the fractions obtained from ovotransferrin by affinity chromatography with Protein G-agarose column: (a) (Peak I) central well: rabbit IgG anti-OVA; well 1: OVA standard wells 2, 3, 4, 5, and 6; (b) (Peak II) central well: rabbit IgG anti-OTf; wells 1, 2, 3, 4, 5, and 6. (c) The peaks of the elution profile obtained after affinity chromatography on Protein G-agarose column: two peaks of elution were obtained by reading fractions at 280 nm: peak I (fractions 3 and 4 with registered values between 0.163 and 0.278 nm) and the fractions of peak II (fractions 7 and 8 with values comprised between 0.275 and 0.469 nm).

(a) Peak I

(b) Peak II

(c)