Chondroprotective Effects and Multitarget Mechanisms of Fu Yuan Capsule in a Rat Osteoarthritis Model

Table 1

RT-PCR primers.

Gene	Sequence
ADAMTS-4
Sense	5′GCCTTTCTCTGGTTTGGAGC3′
Antisense	5′GGCTGGTAATCGGTACAGCA3′
ADAMTS-5
Sense	5′TTCCGTCATTAGCCACAGCAG3′
Antisense	5′GTGTCACAGGTCCTAGAGCAG3′
MMP-1
Sense	5′ACAGTCCATGGATCCAGGTTATC3′
Antisense	5′CGGAGGCTAAATCTGCGTTG3′
MMP-2
Sense	5′GCCCCCATGAAGCCTTGTTT3′
Antisense	5′GCTGGTGCAGCTCTCATACT3′
MMP-3
Sense	5′TGTGGTTGTGTGCTCATCCT3′
Antisense	5′CCTGTCATCTCCAACCCGAG3′
MMP-9
Sense	5′GAAAACCTCCAACCTCACGGA3′
Antisense	5′TTTGGAATCGACCCACGTCT3′
MMP-13
Sense	5′TGAACATCCATCCCGTGACC3′
Antisense	5′ACTCCACACGTGGTTCTCAG3′
IL-1β
Sense	5′ATGCCACCTTTTGACAGTGATG3′
Antisense	5′AGCTTCTCCACAGCCACAAT3′
OPG
Sense	5′GCCACGCAAAAGTGTGGAAT3′
Antisense	5′TTTGGTCCCAGGCAAACTGT3′
RANKL
Sense	5′TCGGGAAGCGTACCTACAGA3′
Antisense	5′CCCCAAAGTACGTCGCATCT3′
TGF-β1
Sense	5′AGCTGCGCTTGCAGAGATTA3′
Antisense	5′AGCCCTGTATTCCGTCTCCT3′
GAPDH
Sense	5′TCAGGAGAGTGTTTCCTCGT3′
Antisense	5′TGCCGTGAGTGGAGTCATAC3′