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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 9086034, 10 pages
Research Article

The Effect of Tong-Xie-Yao-Fang on Intestinal Mucosal Mast Cells in Postinfectious Irritable Bowel Syndrome Rats

1Graduate School, Beijing University of Chinese Medicine, No. 11, Bei San Huan Dong Lu, Chaoyang District, Beijing 100029, China
2Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Haidian District, Beijing 100091, China
3The First Affiliated Hospital of Henan University of TCM, Zhengzhou, Henan 450000, China
4Department of Pharmaceutical Preparation, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Beijing 100091, China

Correspondence should be addressed to Fengyun Wang; moc.361@118yfw and Xudong Tang; moc.anis@yldxt

Received 18 April 2016; Accepted 14 December 2016; Published 26 February 2017

Academic Editor: Jiande D. Z. Chen

Copyright © 2017 Xiangxue Ma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. To investigate the effects of Tong-Xie-Yao-Fang (TXYF) on intestinal mucosal mast cells in rats with postinfectious irritable bowel syndrome (PI-IBS). Design. PI-IBS rat models were established using a multistimulation paradigm. Then, rats were treated with TXYF intragastrically at doses of 2.5, 5.0, and 10.0 g·kg−1·d−1 for 14 days, respectively. Intestinal sensitivity was assessed based on abdominal withdrawal reflex (AWR) scores and fecal water content (FWC). Mast cell counts and the immunofluorescence of tryptase and c-Fos in intestinal mucosa were measured; and serum IL-1β, TNF-α, and histamine levels were determined. Results. AWR reactivity and FWC which were significantly increased could be observed in PI-IBS rats. Remarkably increased mast cell activation ratio in intestinal mucosa, together with increased serum TNF-α and histamine levels, could also be seen in PI-IBS rats; furthermore, PI-IBS-induced changes in mast cell activation and level of serum TNF-α and histamine could be reversed by TXYF treatment. Meanwhile, tryptase and c-Fos expression were also downregulated. Conclusion. TXYF improves PI-IBS symptoms by alleviating behavioral hyperalgesia and antidiarrhea, the underlying mechanism of which involves the inhibitory effects of TXYF on activating mucosal mast cells, downregulating tryptase and c-Fos expression, and reducing serum TNF-α and histamine levels.