Activation of AMPK by Buddleja officinalis Maxim. Flower Extract Contributes to Protecting Hepatocytes from Oxidative Stress

Figure 2

Inhibition of arachidonic acid (AA) + iron-induced cell death by BFE. (a) HepG2 cells were pretreated with 10, 30, and 100 μg/mL of BFE for 1 h and subsequently incubated with 10 μM of AA for 12 h, followed by exposure to 5 μM of iron for 1 h. Cell viability was determined with the MTT assay. (b) Immunoblot analyses of proteins related to apoptosis were performed using HepG2 cell lysates incubated with 10 or 30 μg/mL of BFE for 1 h, continuously treated with 10 μM of AA for 12 h, and then exposed to 5 μM of iron for 1 h. Equal protein loading was verified with β-actin immunoblotting. Data represent the means ± SD of three independent experiments relative to the control ( between the control and AA + iron-treated cells; and between the AA + iron-treated cells without or with BFE).

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