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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 9480791, 6 pages
Research Article

Determination of the Wound Healing Potentials of Medicinal Plants Historically Used in Ghana

1Department of Drug Design and Pharmacology, Universitetsparken 2, 2100 Copenhagen, Denmark
2Museum of Natural Medicine, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark

Correspondence should be addressed to Anna K. Jäger;

Received 12 December 2016; Accepted 16 January 2017; Published 23 February 2017

Academic Editor: Ipek Suntar

Copyright © 2017 Sara H. Freiesleben et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present study was carried out to investigate the wound healing potentials of 17 medicinal plants historically used in Ghana for wound healing. Warm and cold water extracts were prepared from the 17 dried plant species and tested in vitro in the scratch assay with NIH 3T3 fibroblasts from mice. The wound healing scratch assay was used to evaluate the effect of the plants on cell proliferation and/or migration in vitro, as a test for potential wound healing properties. After 21 hours of incubation increased proliferation and/or migration of fibroblasts in the scratch assay was obtained for 5 out of the 17 plant species. HPLC separation of the most active plant extract, which was a warm water extract of Philenoptera cyanescens, revealed the wound healing activity to be attributed to rutin and a triglycoside of quercetin. The present study suggests that Allophylus spicatus, Philenoptera cyanescens, Melanthera scandens, Ocimum gratissimum, and Jasminum dichotomum have wound healing activity in vitro.