Dahuang Fuzi Decoction Attenuates Renal Fibrosis and Ameliorates Mitochondrial Dysfunction in Chronic Aristolochic Acid Nephropathy

Figure 4

Effect of Dahuang Fuzi Decoction (DFD) on kidney ROS production in chronic aristolochic acid nephropathy (AAN) mice. (a) Detection of oxidative products using 2′,7′-dichlorofluorescein (DCF) to measure hydrogen peroxide in the kidney (200). (b) Quantification of the pixel density of DCF staining in glomeruli. (c) Kidney mitochondrial levels of reactive oxygen species (ROS). Data are shown as mean ± SD (). versus control; versus AAN mice by analysis of variance.

(a)

(b)

(c)