Research Article
Aristolochic Acid-Induced Autophagy Promotes Epithelial-to-Myofibroblast Transition in Human Renal Proximal Tubule Epithelial Cells
Table 2
Primary and secondary antibodies for Western blot assay.
| Primary antibody | Cat number | Dilution | Secondary antibody |
| Rabbit anti-Beclin-1 mAb (Cell Signaling) | #3495 | 1 : 1000 | Goat anti-rabbit IgG secondary antibody (LI-COR Biosciences) | Rabbit anti-LC3 mAb (Sigma-Aldrich) | L7543 | 1 : 1000 | Ditto | Mouse anti-E-cadherin mAb (Cell Signaling) | #14472 | 1 : 1000 | Goat anti-mouse IgG secondary antibody (LI-COR Biosciences) | Mouse anti-α-SMA mAb (Sigma-Aldrich) | A2547 | 1 : 1000 | Ditto | Mouse anti--actin mAb (Sigma-Aldrich) | A1978 | 1 : 1000 | Ditto | Mouse anti-fibronectin mAb (Santa Cruz) | sc-8422 | 1 : 1000 | Ditto |
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