Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 9602368, 8 pages
https://doi.org/10.1155/2017/9602368
Research Article

Larvicidal Activity against Aedes aegypti and Chemical Characterization of the Inflorescences of Tagetes patula

1Programa de Pós-Graduação em Ciências Farmacêuticas, Department of Pharmacy, Laboratory of Pharmaceutical Biology (Palafito), Universidade Estadual de Maringá, Avenida Colombo 5790, Maringá, PR, Brazil
2Programa de Pós-Graduação em Agronomia, Department of Agronomy, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Km 380, s/n, Londrina, PR, Brazil
3Department of Animal and Plant Biology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Km 380, s/n, Londrina, PR, Brazil
4Academic Department of Chemistry and Biology, Universidade Tecnológica Federal do Paraná, Linha Santa Bárbara, s/n, Francisco Beltrão, PR, Brazil
5Laboratório de Produtos Naturais e Espectrometria de Massas (LAPNEM), Universidade Federal de Mato Grosso do Sul, Avenida Costa e Silva, s/n, Campo Grande, MS, Brazil
6Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo 5790, Maringá, PR, Brazil

Correspondence should be addressed to João Carlos Palazzo de Mello; rb.meu@ollem

Received 30 June 2017; Accepted 13 November 2017; Published 7 December 2017

Academic Editor: Ghee T. Tan

Copyright © 2017 Letícia Maria Krzyzaniak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The crude acetone extract (CAE) of defatted inflorescences of Tagetes patula was partitioned into five semipurified fractions: n-hexane (HF), dichloromethane (DF), ethyl acetate (EAF), n-butanol (BF), and aqueous (AQF). BF was fractionated by reversed-phase polyamide column chromatography, obtaining 34 subfractions, which were subjected to HSCCC, where patuletin and patulitrin were isolated. CAE and the fractions BF, EAF, DF, and AQF were analyzed by LC-DAD-MS, and patuletin and patulitrin were determined as the major substances in EAF and BF, respectively. BF was also analyzed by HPLC and capillary electrophoresis (CE), and patulitrin was again determined to be the main substance in this fraction. CAE and the semipurified fractions (750, 500, 300, 100, and 50 mg/L) were assayed for larvicidal activity against Aedes aegypti, with mortality rate expressed as percentage. All fractions except AQF showed insecticidal activity after 24 h exposure of larvae to the highest concentration. However, EAF showed the highest activity with more than 50% reduction in larval population at 50 mg/L. The insecticidal activity observed with EAF might have been due to the higher concentration of patuletin present in this fraction.