Research Article

Larvicidal Activity against Aedes aegypti and Chemical Characterization of the Inflorescences of Tagetes patula

Table 1

Eluent systems used for HSCCC to obtain subfractions.

SubfractionEluent systems (v/v)

FB#16hexane : ethyl acetate : methanol : water (2 : 2 : 2.5 : 2)
Gradient elution with n-butanol:
0–400 mL – 0 mL n-butanol
400–800 mL – 20 mL n-butanol
800–1200 mL – 30 mL n-butanol
1200–1400 mL – 40 mL n-butanol

FB#23hexane : ethyl acetate : methanol : water (1 : 5 : 1 : 5)

FB#26hexane : ethyl acetate : methanol : water (2 : 2 : 2.5 : 2)