Protective Effect of Yang Mi Ryung® Extract on Noise-Induced Hearing Loss in Mice

Figure 3

Immunofluorescence phalloidin staining of the organ Corti. (a-b) Hair cells were stained with fluorophore-conjugated phalloidin and observed using a confocal microscope (IX81, Olympus). Immunostaining shows the loss of outer hair cells in the basal turn of cochleae exposed to noise (noise) and the protective effects observed in mice administered with YMRE for 5 weeks (YMRE + noise). (a) Asterisks indicate the areas of the misalignment and hair cell loss. The data are the representative results obtained from three independent experiments. Scale bar 50 μm. Right panels show the representative areas expanded from pictures in the left of each group. (b) The quantification of outer hair cells missing in four cochlear explant groups. The results were represented as mean ± SD, . versus “control” group. versus “noise” group.

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