Research Article

Samul-Tang Regulates Cell Cycle and Migration of Vascular Smooth Muscle Cells against TNF-α Stimulation

Figure 4

Effect of SMT on TNF-α stimulated SMCs secretion and expression of MMPs. Cells were treated with TNF-α (10 ng/ml) for 24 h in the absence or pretreatment of SMT (10, 30, and 50 μg/ml) for 30 min. (a) 0.1% gelatin-containing separating gel was used to measure MMPs secretion in zymography. White bands stand for degraded gelatins by MMP9 and MMP2 secreted in cell cultured medium. (b) Protein expression of MMPs. Bar represents the mean ± SEM of 3 independent experiments. versus untreated control group. versus TNF-α-treated group.