Glehnia littoralis Root Extract Inhibits Fat Accumulation in 3T3-L1 Cells and High-Fat Diet-Induced Obese Mice by Downregulating Adipogenic Gene Expression

Figure 5

Effect of the GLE on the mRNA expression of major adipogenic transcription factors in HFD-induced obese mice. (a) A representative image of the RT-PCR results. mRNA levels of (b) PPARγ, (c) C/EBPα, (d) SREBP-1c, (e) aP2, (f) Leptin, and (g) FAS as determined by RT-PCR. Values are presented as a percentage of the levels in controls. Data are expressed as mean ± SD (). Bars with different superscript letters are significantly different by Duncan’s multiple range test (). Experimental groups: Control, fed basic diet; HFD, fed high-fat diet; HFD + 1% GLE, fed HFD containing 1% GLE; HFD + 5% GLE, fed HFD containing 5% GLE. PPAR, peroxisome proliferator-activated receptor; C/EBP, CCAAT/enhancer-binding protein; FAS, fatty acid synthase; aP2, adipose fatty acid binding protein; SREBP, sterol regulatory element binding protein; RT-PCR, reverse transcription-polymerase chain reaction; GLE, Glehnia littoralis root extract; HFD, high-fat diet.

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