Quercetin Potentiates the NGF-Induced Effects in Cultured PC 12 Cells: Identification by HerboChips Showing a Binding with NGF

Figure 2

(a) Under full scanning mode, the molecular weight of the enriched fraction D1 was shown by LC-MS/MS. (b) ¹H-NMR spectra of enriched fraction D1. (c) ¹³C-NMR spectra of enriched fraction D1. Corresponding chemical structures were indicated according to their ppm in different NMR spectrum. The spectrum was submitted to the database, Biological Magnetic Resonance Data Bank (http://www.bmrb.wisc.edu) for characterization. (a) HPLC chromatograms using the HPLC condition described in the section of Materials and Methods were shown: quercetin at 1 mM. Chemical structure of the enriched fraction from the extract of Ginkgo Folium (D1), that is, quercetin, was shown. (b) Extract of Ginkgo Folium (100 mg/mL) and (c) extract of Ginkgo Folium (50 mg/mL) spiked with 0.5 mM quercetin. The position of quercetin was being indicated in (b) and (c).

(a)

(b)

(c)