ETAS®50 Attenuates Ultraviolet-B-Induced Interleukin-6 Expression by Suppressing Akt Phosphorylation in Normal Human Dermal Fibroblasts

Figure 1

Time course changes in phosphorylation levels of stress-activated MAPKs and Akt after UV-B irradiation in NHDFs. Cells were cultured for 3–24 h after UV-B irradiation (20 mJ/cm²). (Upper panels) phosphorylated and total amounts of JNK subunits p54 (a) and p46 (b), p38 MAPK (c), and Akt (d) were detected by western blotting. (b) An arrow indicates p-p46. Nonspecific, strong luminescence under p-p46 is derived from an unknown protein. (Lower panels) phosphorylation levels were calculated as the ratios of the phosphorylated forms to total amount, mean ± SEM (n = 3). < 0.05 and < 0.01 (by one-way ANOVA and Tukey’s test).

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