ETAS®50 Attenuates Ultraviolet-B-Induced Interleukin-6 Expression by Suppressing Akt Phosphorylation in Normal Human Dermal Fibroblasts

Figure 2

Effects of ETAS 50 treatment on UV-B irradiation-induced phosphorylation of stress-activated MAPKs and Akt in NHDFs. Cells were treated with 1 mg/mL of ETAS 50 or dextrin (vehicle control) for 1 or 3 h after UV-B irradiation (20 mJ/cm²). After 1 h of culture, phosphorylated and total amounts of JNK subunits p54 (a) and p46 (b), as well as p38 MAPK (c), were detected by western blotting; those of Akt (d) were analyzed after 3 h of culture. (b) An arrow indicates p-p46. Nonspecific, strong luminescence under p-p46 is derived from an unknown protein. Phosphorylation levels were calculated as the ratios of the phosphorylated forms to total amount, mean ± SEM (n = 4). < 0.05 and < 0.01 (by one-way ANOVA and Tukey’s test).

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