Cytotoxic Effect of Thymoquinone-Loaded Nanostructured Lipid Carrier (TQ-NLC) on Liver Cancer Cell Integrated with Hepatitis B Genome, Hep3B

Table 1

Cytotoxic effects of TQ and TQ-NLC on the Hep3B and 3T3 reflected by the IC₅₀ values as determined by the MTT assay.

Cell line	Incubation time (hours)	IC50 (μM)
		TQ	TQ-NLC	DMSO	CISPLATIN	NLC
3T3	24	38.1 ± 0.95	34.4±5.92	NP	NP	NP
	48	32.1 ± 5.20	34.4±5.92	NP	NP	NP
	72	31.5 ± 1.70	34.4±5.92	> 50	> 50	> 50
Hep3B	24	16.7 ± 2.86	13.5±3.58	NP	NP	NP
	48	13.1 ± 0.12	11.4±0.72	NP	NP	NP
	72	11.1 ± 0.41	9.20±2.25	> 50	> 50	> 50

Percentage of viable cell computed in comparison to untreated cells calculated as a percentage. Each value was represented as the mean ±SEM. NP: not performed.