Research Article

Cytotoxic Effect of Thymoquinone-Loaded Nanostructured Lipid Carrier (TQ-NLC) on Liver Cancer Cell Integrated with Hepatitis B Genome, Hep3B

Table 2

Percentage of viable, apoptotic, and necrotic Hep3B as determined by flow cytometer.

Percentage of cell
Viable CellEarly apoptosisLate apoptosisNecrosis

UNTREATED89.1 ± 0.84.3 ± 0.73.7 ± 0.12.9 ± 1.1
NLC88.9 ± 2.83.9 ± 1.12.5 ± 1.14.7 ± 2.0
TQ (24 hours)84.5 ± 0.96.0 ± 1.46.9 ± 0.52.6 ± 0.4
TQ (48 hours)83.4 ± 0.47.1 ± 1.36.0 ± 0.93.4 ± 0.3
TQ (72 hours)82.0 ± 3.37.1 ± 0.96.4 ± 0.54.5 ± 1.2
TQ-NLC (24 hours)81.1 ± 1.36.7 ± 1.66.0 ± 0.76.2 ± 2.5
TQ-NLC (48 hours)79.8 ± 3.26.9 ± 1.37.7 ± 0.75.6 ± 0.9
TQ-NLC (72 hours)78.2 ± 2.09.3 ± 0.58.0 ± 1.44.7 ± 0.6

Each data point represents the mean of three independent experiments. Each value was represented as a mean ±SEM.