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Evidence-Based Complementary and Alternative Medicine
Volume 2018, Article ID 1709182, 11 pages
Research Article

Cannabis Essential Oil: A Preliminary Study for the Evaluation of the Brain Effects

1Referring Center for Phytotherapy (CERFIT), Region of Tuscany, Florence, Italy
2Unesco Professorship “Antropologia della Salute-Biosfera e Sistemi di Cura”, University of Genoa, Genoa, Italy
3Center for Integrative Medicine, Careggi University Hospital, Florence, Italy
4Azienda USL 11, Empoli, Italy
5Center IRCCS Don Carlo Gnocchi, Florence, Italy
6Synlab, via della Querciola 12, Sesto Fiorentino, Italy

Correspondence should be addressed to Tania Re; moc.liamg@77er.ainat

Received 18 July 2017; Revised 28 November 2017; Accepted 6 December 2017; Published 17 January 2018

Academic Editor: Orazio Taglialatela-Scafati

Copyright © 2018 Nadia Gulluni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We examined the effects of essential oil from legal (THC <0.2% w/v) hemp variety on the nervous system in 5 healthy volunteers. GC/EIMS and GC/FID analysis of the EO showed that the main components were myrcene and β-caryophyllene. The experiment consisted of measuring autonomic nervous system (ANS) parameters; evaluations of the mood state; and electroencephalography (EEG) recording before treatment, during treatment, and after hemp inhalation periods as compared with control conditions. The results revealed decreased diastolic blood pressure, increased heart rate, and significant increased skin temperature. The subjects described themselves as more energetic, relaxed, and calm. The analysis EEG showed a significant increase in the mean frequency of alpha (8–13 Hz) and significant decreased mean frequency and relative power of beta 2 (18,5–30 Hz) waves. Moreover, an increased power, relative power, and amplitude of theta (4–8 Hz) and alpha brain waves activities and an increment in the delta wave (0,5–4 Hz) power and relative power was recorded in the posterior region of the brain. These results suggest that the brain wave activity and ANS are affected by the inhalation of the EO of Cannabis sativa suggesting a neuromodular activity in cases of stress, depression, and anxiety.