Dandelion Chloroform Extract Promotes Glucose Uptake via the AMPK/GLUT4 Pathway in L6 Cells

Figure 7

DCE-induced GLUT4 translocation was Ca²⁺-independent. (a) The confocal image on the left shows the basic morphology of L6 IRAP-mOrange cells and the fluorescence intensity of intracellular Ca²⁺ at basal level and after treatment with 30 μg/mL DCE for 30 minutes, respectively. The corresponding curve on the right shows that no significant change was seen in the level of intracellular Ca²⁺ in L6 cells after the addition of 30 μg/mL DCE. (b) Changes in IRAP fluorescent intensity at the PM after the addition of 30 μg/mL DCE under 2 mM extracellular Ca²⁺ or 0 mM extracellular Ca²⁺ conditions with 10 μM BAPTA-AM.

(a)

(b)