Inhibitory Effects of Prunella vulgaris L. Extract on 11-HSD1 in Human Skin Cells

Figure 5

Effects of PVE on cortisol levels stimulated with cortisone in HEKn/NHFs coculture. The HEKn were seeded in upper chambers and NHFs were seeded in lower chambers of two-compartment transwell system for 24 h. Upper chambers were pretreated with PVE and 11β-HSD1 inhibitor, BVT.2733, and subsequently with cortisone. The HEKn were placed in the upper chamber with 8 μm-pore filters. (a) Procollagen type I was measured using ELISA. (b) COL1A1 mRNA levels were assayed by quantitative real-time PCR. The results are mean ± standard deviation (SD) (n = 3). P < 0.05 versus cortisone-untreated control, P < 0.05 versus cortisone-treated control, §P < 0.01 versus cortisone-untreated control, P < 0.01 versus cortisone-treated control.

(a)

(b)