An Ethanolic Extract of Allium hookeri Root Alleviates Reflux Esophagitis and Modulates NF-κB Signaling

Figure 1

Safety and anti-inflammatory activities of AHE in LPS-induced RAW264.7 cells. The cells were treated with AHE at various concentrations for 1 h and then stimulated, or not, with LPS for 24 h or 18 h. Cell viability (a) was assayed by the Cell Viability, Proliferation & Cytotoxicity Assay Kit, and the production of NO (b) was measured using the Griess test. The expression levels of iNOS (c), COX-2 (d), and IL-1β (e) in LPS-induced RAW264.7 cells were measured using western blot assay. Values are presented as the mean ± standard deviation of three independent experiments. P<0.001, P<0.01, and P<0.05 versus unstimulated cells; P<0.001, P<0.01, and P<0.05 versus LPS-stimulated cells.

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