Research Article
Phosphatidylinositide 3-Kinase Contributes to the Anti-Inflammatory Effect of Abutilon crispum L. Medik Methanol Extract
Table 1
HPLC condition for analyzing Ac-ME constituents with quercetin, luteolin, and kaempferol as standard compounds.
| Instrument | KNAUER Corp. HPLC system |
| Column | Phenomenex, Gemini 5 μm C18 100A, 250 × 4.60 mm 5 mm | Column temperature | 35°C | Detector | UV/VIS detector 370 nm | Analysis software | ClarityChrom Software | Injection volume | 20 μl | Standard | Diluted with DMSO | Sample treatment | 50 mg/ml, diluted with DMSO | Mobile phase | | Solvent A | 0.1% TFA in H2O | Solvent B | 0.08% TFA in 95% MeCN + 5% H2O | Flow rate | 1.0 ml/min | Time table | Time (min) | Solvent A (%) | Solvent B(%) | | 0 | 100 | 0 | | 50 | 50 | 50 | | 60 | 0 | 100 |
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