Phosphatidylinositide 3-Kinase Contributes to the Anti-Inflammatory Effect of Abutilon crispum L. Medik Methanol Extract

Table 1

HPLC condition for analyzing Ac-ME constituents with quercetin, luteolin, and kaempferol as standard compounds.

Instrument	KNAUER Corp. HPLC system
Column	Phenomenex, Gemini 5 μm C18 100A, 250 × 4.60 mm 5 mm
Column temperature	35°C
Detector	UV/VIS detector 370 nm
Analysis software	ClarityChrom Software
Injection volume	20 μl
Standard	Diluted with DMSO
Sample treatment	50 mg/ml, diluted with DMSO
Mobile phase
Solvent A	0.1% TFA in H2O
Solvent B	0.08% TFA in 95% MeCN + 5% H2O
Flow rate	1.0 ml/min
Time table	Time (min)	Solvent A (%)	Solvent B(%)
	0	100	0
	50	50	50
	60	0	100