Cabbage (Brassica oleracea var. capitata) Protects against H₂O₂-Induced Oxidative Stress by Preventing Mitochondrial Dysfunction in H9c2 Cardiomyoblasts

Figure 2

Cabbage extract inhibits H₂O₂-induced ROS production in H9c2 cardiomyocytes. ROS production was assessed by incubating cells with DCFH-DA for 30 min. (a) Representative images of and (b) fluorescence intensities in H9c2 cardiomyocytes treated with 500 μM H₂O₂ for 24 h following pretreatment with 100, 200, and 300 μg/ml cabbage extract for 24 h. (c) Western blot analysis of SOD-1, catalase, and GPx. (d) Protein expression levels were quantified by scanning densitometry. β-Actin was used as the loading control. Western blot analysis was performed in triplicate with three independent samples. Data are expressed as fold changes ± SEM versus control cells. Significance was analyzed by a one-way ANOVA followed by the Bonferroni post hoc test. ^## P < 0.01 and ^### P < 0.001 versus control cells. P < 0.05, P < 0.01, and P < 0.001 versus H₂O₂ alone-treated cells. Cont, control; Cab, cabbage extract. Scale bar, 100 μm.

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