Standardization of the Manufacturing Process of Bee Venom Pharmacopuncture Containing Melittin as the Active Ingredient

Table 2

Separate substances (detected compound) according to their solvent formulations.

Fr.	Solvent gradient	Amount	Detected compound
	(H2O : ethanol)	(mL)
1	0	50 (each)	ND
2	0		ND
3	0		ND
4	0		ND
5	10%		ND
6	20%		Apamin
7	30%		Apamin, PLA2
8	40%		Apamin, PLA2
9	50%		PLA2
10	60%		PLA2, melittin
11	70%	100	Melittin
12	70%	50	Melittin
13	80%	100	Melittin

ND: not detected.