Xingnaojing Injection Protects against Cerebral Ischemia Reperfusion Injury via PI3K/Akt-Mediated eNOS Phosphorylation

Figure 3

XNJ reduced cerebral ischemia reperfusion injury. (a) Neurologic evaluation. (b) Representative images of coronal brain sections stained with 2,3,5-triphenyltetrazolium chloride. (c) Statistical graph of cerebral infarct size in brains. The percentage of the opposite hemisphere area was used to express the volume of infarction. SD rats were intraperitoneally injected with saline (Sham group and I/R group) or 5 ml/kg, 10 ml/kg, 15 ml/kg XNJ 24 h before the ischemic injury and onset of reperfusion, respectively. Rats underwent 2 h of middle cerebral artery occlusion followed by 24 h of reperfusion. Data were expressed as means ± SD (n = 5-10 separated experiments). p < 0.001 vs. sham-operated group; # p < 0.05 vs. I/R group; ##p < 0.01 vs. I/R group; ###p < 0.001 vs. I/R group.

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