Xingnaojing Injection Protects against Cerebral Ischemia Reperfusion Injury via PI3K/Akt-Mediated eNOS Phosphorylation

Figure 5

Effects of XNJ on the phosphorylations of PI3K (a), Akt308 (b), Akt473 (c), eNOS (d), and NO level (e) in the infarction of rat brain tissues. PI3K, phosphorylated PI3K, Akt, phosphorylated Akt at Thr308 [p-Akt (Thr308)] and Ser473 [p-Akt (Ser473)], eNOS, and phosphorylated eNOS were detected by western blot. The column diagrams show value of p-PI3K relative to that of PI3K, the value of p-Akt relative to that of Akt, and the value of p-eNOS relative to that of eNOS, respectively. NO level was measured by NO kit. Data were expressed as means ± SD (n = 5). p < 0.001 vs. sham group; # p < 0.05 vs. I/R group; ##p < 0.01 vs. I/R group; ###p < 0.001 vs. I/R group.

(a)

(b)

(c)

(d)

(e)