Xingnaojing Injection Protects against Cerebral Ischemia Reperfusion Injury via PI3K/Akt-Mediated eNOS Phosphorylation

Figure 7

PI3K/AKT/eNOS regulated the remission of XNJ on apoptosis in HBMECs. (a) Cells viability was measured by MTT. (b) Apoptotic cells in each group were detected in a flow cytometer. The quantification of annexin V-positive cells (apoptotic cells) was presented in the histogram. (c) Representative fluorescent photographs of cells stained with DAPI and the quantitative statistical charts of the abnormal nuclei ratio. White arrow labeled abnormal (crenation, shrinkage, and fractionation) and normal nuclei. Scale bar = 50 μm. Data were expressed as means ± SD (n = 5). p < 0.001 vs. CTL; #p < 0.05 vs. OGD group; ###p < 0.001 vs. OGD group; &&& p < 0.001 vs. OGD +XNJ (2.5μl/ml) group.

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