Xingnaojing Injection Protects against Cerebral Ischemia Reperfusion Injury via PI3K/Akt-Mediated eNOS Phosphorylation

Figure 8

XNJ alleviated HBMECs apoptosis via regulating caspase activity and mitochondrial potential. (a) The protein expression of procaspase-3 and cleaved caspase-3 was analyzed by western blot. The ratio of cleaved caspase-3/procaspase-3 showed caspase-3 activation. (b) Mitochondrial membrane potential was measured by JC-1 probe. When the mitochondrial membrane potential is high, cells glow with strong red fluorescence; in opposite, cells glow with strong green fluorescence. The relative value of green/red fluorescence indicated the ratio of mitochondrial depolarization in cells. All values came from individual images of each group. Scale bar = 20 μm. Data were expressed as means ± SD (n = 4). p < 0.001 vs. CTL; #p < 0.05 vs. OGD group; ###p < 0.001 vs. OGD group; & p < 0.05 vs. OGD +XNJ (2.5μl/ml) group; &&& p < 0.001 vs. OGD +XNJ (2.5μl/ml) group.

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