Qi-Dong-Huo-Xue-Yin Inhibits Inflammation in Acute Lung Injury in Mice via Toll-Like Receptor 4/Caveolin-1 Signaling

Figure 4

Inhibitory effect of QDHXY on the expression of cytokines in the serum of mice with LPS-induced ALI. Mice were pretreated with QDHXY (0.4 or 0.8 g) or dexamethasone (0.1 mg) daily for 6 days, after which they were treated with 50 μL of 1 mg/mL LPS. The five groups were, respectively, labeled as normal control, ALI, ALI + LDQ (LPS and low-dose QDHXY), ALI + HDQ (LPS and high-dose QDHXY), and ALI + DEX (LPS and dexamethasone). The levels of proinflammatory cytokines [(a) IL-5, IL-7, IL-17; (b) IL-1β; (c) IL-6, G-CSF, GM-CSF; (d) IL-15, TNF-α, IFN-γ; (e) MCP-1, MIP-1α, MIP-1β; and (f) IP-10, KC, RANTES] and anti-inflammatory cytokines [(b) IL-10 and IL-13] in serum were assayed using a MILLIPLEX MAP kit. All data are ratios of a normal control and expressed as mean ± SD of three independent experiments. # indicates < 0.01 and ## indicates < 0.05 when compared to the normal control and ALI groups, respectively.

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