Qi-Dong-Huo-Xue-Yin Inhibits Inflammation in Acute Lung Injury in Mice via Toll-Like Receptor 4/Caveolin-1 Signaling

Figure 5

Effects of QDHXY on caveolin-1, MyD88, and eNOS expression in the lungs of mice with LPS-induced ALI. Mice were pretreated with QDHXY (0.4 or 0.8 g) or dexamethasone (0.1 mg) daily for 6 days, after which they were administered 50 μL of 1 mg/mL LPS via endotracheal instillation. The five groups were, respectively, labeled as normal control, ALI, ALI + LDQ (LPS and low-dose QDHXY), ALI + HDQ (LPS and high-dose QDHXY), and ALI + DEX (LPS and dexamethasone). Lung tissues were studied immunohistochemically. Changes in the expression levels of (a) caveolin-1, (b) MyD88, and (c) eNOS in the lung were observed under a microscope (original magnification: ×400). The positively stained cells are seen as yellow or brown granules in the cytoplasm and nuclei. (d) IOD/area of caveolin-1, MyD88, and eNOS in the lung were calculated. Data were analyzed using Image-Pro Plus software. All data are ratios of the normal control and expressed as mean ± SD of three independent experiments. # indicates < 0.01 and ## indicates < 0.05 when compared to the normal control and ALI groups, respectively.

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