Neuroprotective Effects of Gagam-Sipjeondaebo-Tang, a Novel Herbal Formula, against MPTP-Induced Parkinsonian Mice and MPP⁺-Induced Cell Death in SH-SY5Y Cells

Figure 2

Effects of GST extract on MPP⁺-induced cell death in SH-SY5Y cells. (a) Effects of GST extract on SH-SY5Y cell viability. Cells were treated with various concentrations of GST extract (10, 30, 50, 100, 300, or 500 μg/ml) for 24 h. Cell viability was determined by MTT assay. Values were expressed as percentages of the control. (b) Effects of GST extract against MPP⁺-induced neurotoxicity in SH-SY5Y cells. SH-SY5Y cells were pretreated with GST (10, 100, 200, or 300 μg/ml) for 1 h and then exposed to 1 mM MPP⁺ for 24 h. The cell viability was measured using an MTT assay. ### p < 0.001, compared to control group; ∗∗∗ p < 0.001, compared to MPP⁺ treatment, analyzed by one-way ANOVA, followed by Bonferroni’s post hoc test. (c) Effects of GST on MPP⁺-induced apoptosis in SH-SY5Y cells. The expression of apoptosis-related proteins were measured by western blot in cells treated with 100 μg/ml GST in the absence or presence of 1 mM MPP⁺ for 24 h. (d) Effects of GST on MPP⁺-induced changes of cell signaling proteins in SH-SY5Y cells. The expression of cell proliferation-related proteins was assayed by western blot in cells treated with 100 μg/ml GST in the absence or presence of 1 mM MPP⁺ for 24 h.

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