QiDiTangShen Granules Reduced Diabetic Kidney Injury by Regulating the Phosphorylation Balance of the Tyrosine and Serine Residues of Insulin Receptor Substrate 1

Figure 5

QDTS granules reduced phosphorylation of the serine residues of IRS-1 and restored the phosphorylation balance of tyrosine/serine residues of IRS-1 in kidney tissues of the db/db mice. (a) Expression of IRS-1. (b) Expression of phosphorylated Tyr896 of IRS-1. (c) Expression of phosphorylated Ser302 of IRS-1. (d) Ratio of phosphorylated Ser302/Tyr896 of IRS-1. (e) Expression of phosphorylated Ser307 of IRS-1. (f) Ratio of phosphorylated Ser307/Tyr896 of IRS-1. (g) Western blot of the expression of IRS-1 and the phosphorylated tyrosine and serine residues of IRS-1. Lane 1, the C57BL/6 group; Lane 2, the db/db model group; Lane 3, the db/db + v group; Lane 4, the db/db + Q group. C57BL/6, the normal control group; db/db, the diabetic nephropathy (DN) model group; db/db + v, the valsartan intervention group; db/db + Q, the QDTS granules intervention group. n = 4 for each group. P < 0.05 compared with the normal control, P < 0.01 compared with the normal control, P < 0.05 compared with the DN model group, and P < 0.01 compared with the DN model group.

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