Tetrahydroxystilbene Glucoside (TSG) Restores the Effect of Transient Hypoxia on Reperfusion Injury in Senescent H9c2 Cells by Regulating Mitochondrial Energy Metabolism

Figure 1

TSG restored protective effect of transient hypoxia (TH) on senescent H9c2 cells against HR injury. (a) Effect of TH and TSG on cell viability in senescent H9c2 cells against HR injury determined by MTT assay. Data (n=5) were shown as the mean ± SEM, p<0.05 and p<0.01, p<0.001. (b) Effect of TH and TSG on mitochondrial viability in senescent H9c2 cells against HR injury determined by Mitochondrial Viability assay. Data (n=5) were shown as the mean ± SEM, p<0.001. (c, d) Effects of TH and TSG on ROS production and Ca²⁺ concentration in senescent H9c2 cells against HR injury determined by flow cytometry, respectively, stained by H₂DCFDA and Fluo-4, AM. Data (n=3) were shown as the mean ± SEM, p<0.05, p<0.01, p<0.001.

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