Bushenhuoxue Formula Facilitates Articular Cartilage Repair and Attenuates Matrix Degradation by Activation of TGF-β Signaling Pathway

Figure 4

(a) IHC for pSMAD2, Bar=200μm, black arrows point out the positive cells. (b) pSMAD2 positive cell ratio. Transfection efficiency of Tgfβr2 siRNA in Chondrogenic ATDC5 cells. (c) After transfected and BSHX formula treatment, ECM deposition (d), matrix degradation (e), and chondrocytes hypertrophy (f) related gene expression alterations in chondrogenic ATDC5 cells. (g) The protein expression of pSMAD2/3 in chondrogenic ATDC5 cells. P<0.05, #P<0.01.

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