Bushenhuoxue Formula Facilitates Articular Cartilage Repair and Attenuates Matrix Degradation by Activation of TGF-β Signaling Pathway

Table 2

Primer name and sequences for PCR analysis.

Primer name	Sequence
β-actin forward	5′-GGAGATTACTGCCCTGGCTCCTA-3′
β-actin reverse	5′-GACTCATCTACTCCTGCTTGCTG-3′
Col2a1 forward	5′-TGGTCCTCT GGGCATCTCAGGC-3′
Col2a1 reverse	5′-GGTGAACCTGCTGTTGCCCTCA-3′
Mmp13 forward	5′-TTTGAGAACACGGGGAAGA-3′
Mmp13 reverse	5′-ACTTTGTTGCCAATTCCAGG-3′
Aggrecan forward	5′-CGCCACTTTCATGACCGAGA-3′
Aggrecan reverse	5′-TCATTCAGACCGATCCACTGGTAG-3′
Adamts5 forward	5′-CCAAATGCACTTCAGCCACGATCA-3′
Adamts5 reverse	5′-AATGTCAAGTTGCACTGCTGGGTG-3′
Col10a1 forward	5′-ACCCCAAGGACCTAAAGGAA-3′
Col10a1 reverse	5′-CCCCAGGATACCCTGTTTTT-3′
Runx2/Cbfa1 forward	5′-GAGGGCACAAGTTCTATCTGGA-3′
Runx2/Cbfa1 reverse	5′-GGTGGTCCGCGATGATCTC-3′