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Evidence-Based Complementary and Alternative Medicine
Volume 2018, Article ID 2738489, 11 pages
https://doi.org/10.1155/2018/2738489
Research Article

Seasonal Effect on the Biological Activities of Litsea glaucescens Kunth Extracts

1Centro de Investigación en Alimentación y Desarrollo (CIAD), 83000 Hermosillo, SON, Mexico
2Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Rosales y Luis Encinas, 83000 Hermosillo, SON, Mexico
3Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., CONSORCIO-ADESUR, Av. Normalistas 800, Colinas de la Normal, 44270 Guadalajara, JAL, Mexico
4Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Clúster Científico y Tecnológico Biomimic®, Carretera Antigua a Coatepec No. 351, Colonia El Haya, 91070 Xalapa, VER, Mexico
5Red de Estudios Moleculares Avanzados, Clúster Biomimic®, Instituto de Ecología, A. C., Carretera Antigua a Coatepec No. 351, Colonia El Haya, 91070 Xalapa, VER, Mexico
6Unidad de Servicios de Apoyo en Resolución Analítica, Universidad Veracruzana, 575 Xalapa, VER, Mexico

Correspondence should be addressed to Zaira Domínguez; xm.vu@zeugnimodz and Javier Hernández; xm.vu@zenitramvaj

Received 14 October 2017; Revised 14 January 2018; Accepted 22 January 2018; Published 20 February 2018

Academic Editor: Letizia Angiolella

Copyright © 2018 Julio César López-Romero et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study shows the seasonal effect on the antioxidant, antiproliferative, and antimicrobial activities of L. glaucescens Kunth (LG) leaves extracts. Their antioxidant activity was evaluated through the DPPH, FRAP, and ORAC assays. Their phenolic content (PC) was determined by means of the Folin-Ciocalteu method, and the main phenolic compounds were identified through a HPLC-DAD analysis. Antiproliferative activity was determined by MTT assay against HeLa, LS 180, M12.C3.F6, and ARPE cell lines. Antimicrobial potential was evaluated against Staphylococcus aureus and Escherichia coli using a microdilution method. All the LG extracts presented high antioxidant activity and PC, with quercitrin and epicatechin being the most abundant. Antioxidant activity and PC were affected by the season; particularly autumn (ALGE) and summer (SULGE) extracts exhibited the highest values (). All extracts presented moderate antiproliferative activity against the cell lines evaluated, HeLa being the most susceptible of them. However, ALGE and SULGE were the most active too. About antimicrobial activity, SULGE (μg/mL; MIC50 < 400 μg/mL), and SLGE (MIC50 < 1000 μg/mL) showed a moderate inhibitory effect against S. aureus. These findings provide new information about the seasonal effect on the PC and biological properties of LG extracts. Clearly, antioxidant activity was the most important with respect to the other two.