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Evidence-Based Complementary and Alternative Medicine
Volume 2018, Article ID 2907812, 7 pages
https://doi.org/10.1155/2018/2907812
Research Article

Analysis of Dietary Related Factors of Recurrent Aphthous Stomatitis among College Students

1Beijing University of Chinese Medicine, Beijing 100029, China
2Wenzhou Traditional Chinese Medicine Hospital, Zhejiang 325000, China

Correspondence should be addressed to Yanling Fu; nc.ude.mcub@gnilnayuf

Received 18 October 2017; Accepted 31 January 2018; Published 27 February 2018

Academic Editor: Cleofas Rodríguez-Blanco

Copyright © 2018 Qian Du et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. We investigated the occurrence of recurrent aphthous stomatitis (RAS) among college students and its potential influence by dietary habits. Methods. Study of dietary habits and RAS among students in Beijing University of Chinese Medicine was carried by homemade questionnaire. Multivariate binary logistic regression analysis was used to identify RAS risk factors and explore their relations. Results. Among 1011 investigated college students, family history (odds ratio (OR) 1.678, 95% confidence intervals (CI) 1.192 to 2.364, ), bed late (OR 1.515, 95% CI 1.005 to 2.285, ), frequent thirst (OR 1.842, 95% CI 1.393 to 2.435, ), and frequent drinking carbonated beverages (OR 1.369, 95% CI 1.029 to 1.821, ) were independent risk factors for RAS, but preference for nuts (OR 0.607, 95% CI 0.448 to 0.824, ) was a protective factor. There was no statistical difference in fruit intake between RAS and non-RAS groups (χ2 = 5.249, ). Conclusions. Among college students, frequent drinking carbonated beverages or frequent thirst will increase its possibility, whereas preference for nuts provides protection. In addition, fruit intake does not have a positive effect.

1. Introduction

Recurrent aphthous stomatitis (RAS), or recurrent oral ulceration (ROU), is one of the most common oral mucosal diseases. According to worldwide epidemiological data, 2%–66% of the international population is affected [1, 2]. The prevalence of RAS is about 20% in China. The cause of the disease is complicated by its periodicity. In modern medicine, many factors including heredity, oral microbiome disorders, immunologic abnormalities, microcirculatory disturbances, trace element deficiencies, endocrine dyscrasia, and gastrointestinal dysfunction are related to RAS occurrence [37], but, to date, the exact etiology and pathogenesis are still unclear. With the change of the whole medical model to preventive medicine, there is an urgency in exploring the diet to interfere with the onset of the disease. Some scholars suggest that the incidence of RAS is closely related to diet, and dietary control has a good application value in its remission by effectively guiding the treatment [8, 9].

In previous studies of RAS relating to diet, some have suggested that spicy food and fried food are risk factors of RAS in the research on the relationship between RAS and bedtime or other pathogenic risk factors [10, 11]. Some scholars choose one or several allergic food(s) in RAS patients by intake or fast test to verify the correlation between RAS and these selected allergic foods [1214]. Other research focuses on the beneficial effects on RAS by trace elements such as vitamin and dietary fiber and draws a conclusion that more intake of these trace-element-containing food items including grain, dairy product, and fruit can prevent or treat RAS [15]. These studies mentioned above only involve some or some kind of food, and systematic research on daily dietary habits in RAS, especially the possible difference in daily drinks including water intake or beverage, is rarely referred. It is noticed that both milk and grains have been confirmed to cause allergic reactions including RAS [16, 17]. Therefore, it is of great importance to systematically investigate the dietary related factors with RAS.

This study mainly focuses on investigating the prevalence of RAS among college students in Beijing University of Chinese Medicine, analyze the difference of daily dietary habits between RAS patients and the healthy people, and explore its potential influence on RAS. It is beneficial to provide some basis for RAS prevention and treatment in medical students and some guidance in the clinic in future.

2. Methods

2.1. Subjects

Sixty college students were randomly selected to fill in the self-designed questionnaire after informed consent as a preinvestigation. The prevalence of RAS in preinvestigation was 30%. Based on the formula for estimating sample size, 900 is the baseline. Assume that the nonresponse rate is 10%, 1000 respondents will be suitable. The formula is as follows:where represents the size of sample, is the statistic in confidence level , is the expected proportion of target population, and is half width of confidence interval (error survey, 0.1-fold of ).

2.2. Questionnaire

All questions in this questionnaire (see Supplementary Materials (available here)) are based on their own conditions in recent one year. The questionnaire can be divided into three parts, suffering from RAS or not, underlying RAS risk factors, and daily dietary habits. Fifty items are included in this questionnaire. In basic information, age, gender, height, weight, and grade are involved. In Part I, RAS or not, prevalence, therapeutic methods, and remission, and food or flavor worsening or triggering RAS are included. In Part II, wearing dental supplier, family history, allergic history, gastrointestinal disorders, tooth brushing, sleeping, mental status, flavor preference for food, drinking water in warm or cold, dishes mix, and three-meal taking are included. In Part III, habits of taking coffee, tea, alcohol, sweet drink, carbonated beverages, milk, fried food, dessert, ice cream, and fruit are investigated. Exclusion criteria are ① illogical or paradoxical data; ② arbitrary ticking, for instance, ticking the first choice in all items; ③ not answering five or over five items.

2.3. Discrimination of RAS

We judge the oral ulceration to be RAS based on its clinical characteristics including periodicity, affected area, and self-limitation. The severity of RAS is graded as the frequency, amount, size, and duration of the ulceration [18].

2.4. Cross-Sectional Investigation

The survey was lasted 2 weeks from July 1 to 14, 2017. A total of 1011 college students from Beijing University of Chinese Medicine were enrolled in this study. Stratified sampling and systematic random sampling were successively used. After calculation, there were about 10,000 college students with the ratio of male to female being 9 : 16. The sample proportion was 0.1. The sample size of male and female was 360 and 640, respectively. Four students were classified into a dormitory. The dormitory number was coded at a distance of 10 during sampling. These investigators were all trained about the purpose, significance, and methods of filling in the questionnaire. These questionnaires were collected when the investigators were on the spot in order to deal with doubts in time.

2.5. Statistical Analysis

The original data were input into Excel and analyzed by SPSS 20.0. All the data were separately recorded by Qian Du and Sanhai Liu for correction. Based on the acquired literature and outcome in preinvestigation, every risk factor related to RAS was put through binary logistic forward regression analysis to screen out the independent risk factors and control the confounding bias. The regression fitness was judged by Hosmer-Lemeshow test, and the correlation was evaluated by odds ratio (OR) and 95% confidence interval (CI). In Part III, the difference of specific dietary habits in RAS and non-RAS was compared by a chi-square (χ2) test. The frequency of food or flavor triggering oral ulceration was calculated. Three conditions of fruit intake during ulceration were calculated in their proportions and the difference was analyzed by a chi-square test. was considered as statistically significant.

2.6. Ethics

The study protocol was approved by the Ethics Committee of Beijing University of Chinese Medicine (number 2017BZHYLL0308). All participants were informed of the purpose, general contents, and data use.

3. Results

3.1. Characteristics of the Participants

The number of effective questionnaires was 1011, and the recovery rate was 99.6%. The proportion of male to female was 367 to 644. There were 297 college students with RAS accounting for 29.38%. Among these non-RAS participants, 465 students suffered from oral ulceration but could not be diagnosed as RAS and 249 healthy ones did not have oral ulceration. For RAS students, there was no statistical difference in gender, body mass index (BMI), or grade (Table 1).

Table 1: General characteristics of 1011 college students.
3.2. Risk Factors Analysis of RAS

All factors including gender, BMI, age, family history, wearing dental braces, gastrointestinal diseases, times of brushing, bedtime at night, duration of brushing time, daily sleep duration, common cold, stress, regular diet, drinking water, and dishes mix were designed as the independent variables. In order to control the confounding bias of dietary factors, dietary contents in Part III were performed with univariate analysis. Besides, frequent intake of sweet drinks, carbonated beverages, and fried food, and preference for nuts which displayed statistical significance was also put into the independent variables for multivariate binary logistic regression analysis. The logistic model was statistically significant (; ). Forward (LR) regression indicated that family history, bedtime later than 11 P.M., frequent thirst, frequent consumption of carbonated drinks, and preference for nuts displayed statistical significance. The results showed that the risk of people with family history of RAS was 1.678 times compared with that without RAS family history. People who slept after 11 P.M. suffering from RAS were 1.515 times as high as those sleeping before 11 P.M. For those always felt thirsty, the risk of RAS was 1.842 times in comparison with those who were absent. The risk of RAS was increased by 31.4% compared with those who seldom drank carbonated beverages. People preferring for nuts had a 49.9% lower risk of RAS than those who do not. The goodness of fit was 0.572 in the regression model with a favorable outcome (Table 2).

Table 2: Multivariate binary logistic regression of RAS.

Table 3 showed that, with regard to daily dietary habits between RAS and non-RAS, there were statistical differences in taking fried food, sweet and carbonated beverage intake, and preference for nuts (all ). The frequency of food intake or beverage consumption was divided into 5 grades including daily, often, sometimes, seldom, and hardly. Here, “more” refers to the sum of the first three grades, and “less” indicates the last two ones.

Table 3: Frequency or hobby of food intake.
3.3. Influence of Food and Flavor on RAS

A previous study suggested that some food might trigger or worsen oral ulceration [19]. Therefore, according to clinical practice and outcome in preinvestigation, 7 daily food and five flavors were selected. The subjects were composed of 297 RAS and 465 students who had oral ulceration in recent one year. Based on their own conditions, after analysis, the top three foods triggering or worsening oral ulceration were pineapple (291/762), lemon (182/762), and vinegar (160/762). The food flavor was displayed from high to low as acrid (spicy) > sour > salty > sweet > bitter accordingly (Figure 1).

Figure 1: Food or flavor triggering/aggravating RAS.

Furthermore, we compared the selection differences of pineapple and spicy (flavor) between RAS group and non-RAS group. There was no significant difference in pineapple or spicy (flavor) selection between the two groups (both ). The result displayed that pineapple and spicy (flavor) were considered to trigger or worsen oral ulceration in the two groups (Table 4).

Table 4: Pineapple and spicy food triggering/aggravating RAS or not.

In addition, we found an interesting phenomenon during onset of oral ulceration-some ate more or less fruits on purpose, and others did not care about this. Among these 762 college students with oral ulceration, the number of eating more and less fruits was 401 (52.6%) and 47 (6.2%), respectively. The rest 314 students did not care about the influence of eating fruits on oral ulceration (Table 5).

Table 5: Comparison of fruit intake during oral ulceration between RAS group and non-RAS group.

After a chi-square test, no statistical difference was displayed ; , which indicated no great difference in fruit intake by the three ways during the onset of oral ulceration between RAS group and non-RAS group. It is not conclusive that fruit intake has an active effect on oral ulceration healing. With reference to the result of frequency of daily fruit intake in Table 3, the conclusion that fruit intake decreases the prevalence of RAS cannot be drawn.

4. Discussion

Recurrent aphthous stomatitis is categorized into oral ulcer (kou chuang) or oral erosion (kou mi) in traditional Chinese Medicine, which was recorded in Internal Canon of Huangdi. Oral diseases are directly associated with dietary factors. We endeavor to explore the correlation between RAS and dietary factors.

4.1. Prevalence of RAS

Our study showed that the prevalence of RAS in college students from Beijing University of Chinese Medicine (North China) was 29.38%. Zhong et al. report the RAS prevalence in college students in Xinjiang Medical colleges (Northwest, China) was 13.1% in 2014 [20]. Shi et al. report the RAS prevalence in college students in Wuhu City (East China) in 2015 was 23.30% [11]. In Sichuan University (Southwest, China), RAS prevalence in 2014 reported by Ma et al. was 53.2% [10]. The RAS prevalence was 59.43% in college students in Guangzhou Medical College (South China) by Xie et al. in 2009 [21]. The above research indicated that RAS prevalence in college students in Beijing University of Chinese Medicine was high, and the oral health status was not satisfactory. RAS prevalence was varied in different regions in China as a sequence of South China, Southwest, North China, East China, and Northwest. It was assumed that difference RAS prevalence might be associated with geographical and environmental factors and dietary factors. But in Northwest regions, more occurrences of dry mouth and thirst but with a lower RAS prevalence could be explained as drinking more water, which still needs further confirmation. Some research has proved bad habit of tooth brushing and stress were risk factors of RAS [22, 23], but no great difference between RAS group and non-RAS group, which suggested that oral disease prevention and health education in college student should be intensified.

4.2. Correlation between RAS and Dietary Factors

Our finding displayed that frequent consumption of carbonated beverages was an independent risk factor for RAS. The prevalence of RAS was higher in those who frequent drinking of sweet drinks, carbonated beverages, and higher intake of fried foods. The habits of sweet and acidic intake can lead to changes in pH in the mouth. Normally, the saliva is neutral and can maintain the stability of the oral chemical environment, while sweet or carbonated beverages lower the pH and change the oral environment. It is reported that stomatitis is more likely to occur when pH in the mouth is abnormal [24]. Teeth invasion by soft drink leads to excessive detrition of dental enamel. Carbonated drink softens the enamel surface leading to extremely rough, porous, and alveolate demineralization, which then causes the wear of soft tissues in the mouth [2527]. At the same time, it is displayed that preference for nuts is a protective factor for RAS, which could be explained by nuts being rich in vitamins A, B, and E and proteins. If in shortage, they will trigger RAS [28]. Additionally, antioxidant components including flavone and phenolic acids (tannin) in nuts can clear free radicals and have effects of antisepsis and anti-inflammation. Unsaturated fatty acids in nuts may have some lubrication effect on the oral mucosa and reduce the incidence of RAS [29]. Fried and spicy foods are in high calories resulting in a temporary shortage of free moisture in the mouth, which disturbs the balance between free water and bound water in the surface of oral mucosa, intensifying the energy metabolism by heat increase, and symptoms of uprising of fire including aphtha ensue [30]. Reduction of saliva in the mouth fails in protection of oral mucosa. Spicy food has a stimulating effect on the oral mucosa. According to the data in Figure 1, pineapple may induce or aggravate oral ulcers, which may be related to glycosides in the pineapple stimulating oral mucosa and protease leading to allergic reactions in some people [31, 32].

4.3. Correlation between RAS and Dry Mouth

This study revealed that people who often present with dry mouth and thirst are more prone to develop RAS. Through clinical trials, Gu et al. found that the secretion by salivary glands in RAS patients is inferior to that in the healthy, and RAS is in spontaneous recovery when secretion becomes normal [33]. It is indicated that the protective characteristics of saliva are not limited to the traditional concepts of cleaning, washing, lubrication, buffering, and supplementing minerals, but also in the transportation of a variety of antiviral, antibacterial, and antifungal factors, and polypeptide growth factors. From this, great attention should be paid to research on salivary secretion in the clinical treatment of RAS.

Usually, people believe oral ulceration to be due to uprising of fire and thirst and preference for cold drinks which are common symptoms. But in our investigation, among RAS students, some students were absent of thirst. Some were thirsty and preferred drinks. Others felt thirsty but were reluctant to have a drink. For those who were thirsty and preferred drinks, the drinks could be divided into hot and cold, which indicates cold and heat patterns, respectively, from the perspective of eight-principle pattern identification. This is helpful for RAS prevention and treatment by traditional Chinese medicine.

4.4. Correlation of RAS and Fruits

Our study revealed that, in both RAS and non-RAS groups, daily consumption of fruits was high (Table 3). In traditional Chinese medicine, it is deemed that fruits have cold or heat properties. People with cold deficiency constitution will worsen their conditions by eating cold fruits, while, for those with a heat constitution, taking fruits in heat property will aggravate the symptoms including oral ulcers. Based on Table 5, 52.6% participants will take more fruits on purpose during oral ulceration, which may be related to the idea of rich vitamins in fruits being effective on ulcer healing or intake of more fruits being able to subdue fire and supplement water for oral ulcer being caused by fire [34], but these views are not entirely correct. Lalla et al. confirmed that daily intake of multivitamin as a supplementation could reduce the frequency or duration of RAS attack by a randomized, double-blind trial. It is suggested that multivitamin as a supplement should not be used on RAS prevention by clinical physicians [35]. This can be verified by pineapple triggering or aggravating oral ulcer in our study. The etiology of oral ulcer is related to not only fire, but cold as well. Therefore, in the treatment of RAS, sole focus on selecting herbs with effects of clearing heat, dispersing fire, and resolving toxins is not entirely appropriate, as formulas in warming and supplementing is also useful in clinic [36, 37]. Meanwhile, fruit intake during oral ulcer should be done with caution.

5. Conclusions

Our findings indicate that family history, bedtime later than 11 P.M., dry mouth and thirst all the time, and frequent intake of carbonated beverages were independent risk factors of RAS. It is suggested that college students, especially those suffering from RAS, should pay attention to the family history and seek treatment. It is advised not to stay up late. In the aspect of dietary habits, frequent water drinking, reduction of intake on carbonated drinks, and slight increase of nuts intake are advocated. At the same time, fried food and sweet drinks should be reduced. Large quantities of fruits intake do not play a positive role in the prevention and treatment of RAS, so one should be cautious on ingestion and selection of fruits. Pineapple and spicy foods may stimulate or aggravate oral ulcers, people with RAS or during oral ulceration are best to avoid these foods.

Additional Points

Limitation. We only focused on college students from bachelor to doctor degrees in one university in Beijing without considering the age factor. The outcomes were obtained based on the data collected in July 2017 (in summer). No other relative documents referring to the influence of different seasons on RAS were reported. Therefore, one should be cautious when applying our outcomes to explain RAS during other seasons. In addition, large-scale social survey besides college students is necessary for comprehensive exploration of RAS’s current status and corresponding therapeutic measures.

Disclosure

Shenglou Ni is co-first author.

Conflicts of Interest

The authors declare that there are no conflicts of interest.

Authors’ Contributions

Qian Du and Shenglou Ni designed the questionnaire. Yanling Fu revised the questionnaire and provided valuable suggestions on the investigation. Qian Du and Sanhai Liu recorded and analyzed the data. Qian Du wrote the manuscript and Shenglou Ni assisted in the further revision. All authors read and approved the final manuscript.

Acknowledgments

The authors are grateful to Associate Researcher Yutong Fei, Professor Jian Zhou, and Associate Professor Xuefeng Shi from Beijing University of Chinese Medicine for their professional suggestions on the aspects of evidence-based medicine, nutriology, and statistics. Great thanks are due to their teammate of Wenjie Song, Xinrong Wang, Xun Ma, Ni Liu, Yang Liu, Jiajia Fan, Xiangjun Pei, Xianzheng Wang, Kun Zhao, Wenchao Zhang, Fanhui Jin, Chuanlei Liang, Yanlin Liang, Luqi Wang, and Zhigui Huang for helping to deliver and collect the questionnaires. During data analysis, the authors also got help from Mei Han and Hui Luo from Beijing University of Chinese Medicine. In addition, they express their gratitude to Quanming Tan (Thong Chai Medical Institution, Singapore) for his final language polishing.

Supplementary Materials

Fifty items including general demographic information, dietary habits, life style, and so forth on RAS were investigated in this questionnaire. (Supplementary Materials)

References

  1. S. Hamedi, O. Sadeghpour, M. R. Shamsardekani, G. Amin, D. Hajighasemali, and Z. Feyzabadi, “The most common herbs to cure the most common oral disease: stomatitis recurrent aphthous ulcer (RAU),” Iranian Red Crescent Medical Journal, vol. 18, no. 2, Article ID e21694, 2016. View at Publisher · View at Google Scholar · View at Scopus
  2. D. E. A. Wardhana, “Recurrent aphthous stomatitis caused by food allergy,” Acta Medica Indonesiana—The Indonesian Journal of Internal Medicine, vol. 42, no. 4, pp. 236–240, 2010. View at Google Scholar
  3. J. Han, Z. He, K. Li, and L. Hou, “Microarray analysis of potential genes in the pathogenesis of recurrent oral ulcer,” International Journal of Clinical and Experimental Pathology, vol. 8, no. 10, pp. 12419–12427, 2015. View at Google Scholar · View at Scopus
  4. S. S. Natah, Y. T. Konttinen, N. S. Enattah, N. Ashammakhi, K. A. Sharkey, and R. Häyrinen-Immonen, “Recurrent aphthous ulcers today: a review of the growing knowledge,” International Journal of Oral and Maxillofacial Surgery, vol. 33, no. 3, pp. 221–234, 2004. View at Publisher · View at Google Scholar · View at Scopus
  5. N. Jiang, L. Luo, L. Liu, X. Q. Sun, X. Jiang, and Y. Cai, “Soluble programmed death-1 and soluble programmed death ligand 1 protein expression and immune status in patients with recurrent aphthous ulcer,” West China Journal of stomatology, vol. 35, no. 3, pp. 286–290, 2017. View at Google Scholar
  6. J. Chen and W. J. Ding, “Correlation between microbial and immune mechanisms of recurrent oral ulcer and traditional Chinese medicine,” Chinese Journal of Experimental Traditional Medical Formulae, vol. 22, no. 13, pp. 202–207, 2016. View at Google Scholar
  7. Z. X. Wang, “Epidemiological analysis of gastrointestinal diseases and recurrent oral ulcers,” General Journal Of Stomatology, vol. 3, no. 3, p. 148, 2016. View at Google Scholar
  8. Q. L. Cai, M. Y. Chen, and Z. C. Mo, “The current status of the relationship between the recurrent aphthous ulcer and the diet,” Studies of Trace Elements and Health, vol. 26, no. 4, pp. 58–61, 2009. View at Google Scholar
  9. B. Tarakji, K. Baroudi, and Y. Kharma, “The effect of dietary habits on the development of the recurrent aphthous stomatitis,” Nigerian Medical Journal, vol. 53, no. 1, pp. 9–11, 2012. View at Publisher · View at Google Scholar
  10. R. Ma, H. Chen, T. Zhou et al., “Effect of bedtime on recurrent aphthous stomatitis in college students,” Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, vol. 119, no. 2, pp. 196–201, 2015. View at Publisher · View at Google Scholar · View at Scopus
  11. L. Shi, K. Wan, M. Tan et al., “Risk factors of recurrent aphthous ulceration among university students,” International Journal of Clinical and Experimental Medicine, vol. 8, no. 4, pp. 6218–6223, 2015. View at Google Scholar · View at Scopus
  12. X. H. Pan, H. Sun, Q. Chen, J. Y. Ding, and W. L. Gu, “Clinical research for alimentary control of certain foods to remission of recurrent oral ulcer,” West China Journal of Stomatology, vol. 28, no. 1, pp. 68–70, 2010. View at Google Scholar
  13. N. Chainani-Wu and A. Nayudu, “Resolution of recurrent aphthous ulcers after discontinuation of cow's milk protein intake,” The Journal of the American Dental Association, vol. 148, no. 8, pp. 614–617, 2017. View at Publisher · View at Google Scholar · View at Scopus
  14. D. Gülseren, A. Hapa, S. Ersoy-Evans, G. Elçin, and A. Karaduman, “Is there a role of food additives in recurrent aphthous stomatitis? A prospective study with patch testing,” International Journal of Dermatology, vol. 56, no. 3, pp. 302–306, 2016. View at Publisher · View at Google Scholar · View at Scopus
  15. Y. P. Sun, “Clinical study of dietary control to alleviate recurrent aphthous ulcers,” General Journal Of Stomatology, vol. 3, no. 18, pp. 37-38, 2016. View at Google Scholar
  16. I. Besu, L. Jankovic, A. Konic-Ristic, A. Damjanovic, V. Besu, and Z. Juranic, “Good tolerance to goat's milk in patients with recurrent aphthous ulcers with increased immunoreactivity to cow's milk proteins,” Journal of Oral Pathology & Medicine, vol. 42, no. 7, pp. 523–527, 2013. View at Publisher · View at Google Scholar · View at Scopus
  17. E. A. Sabel'Nikova, L. M. Krums, A. I. Parfenov et al., “Gluten-sensitive celiac disease associated with recurrent aphthous stomatitis and schizophrenia,” Terapevticheskii Arkhiv, vol. 85, no. 2, pp. 54–56, 2013. View at Google Scholar
  18. K. L. Wei, Diseases of the Oral Mucosa, Science Press, Beijing, China, 2006.
  19. A. Altenburg, N. El-Haj, C. Micheli, M. Puttkammer, M. B. Abdel-Naser, and C. C. Zouboulis, “The treatment of chronic recurrent oral aphthous ulcers,” Deutsches Ärzteblatt International, vol. 111, no. 40, pp. 665–673, 2014. View at Publisher · View at Google Scholar · View at Scopus
  20. L. Zhong, D. L. Huang, H. Wang, and Y. P. Sun, “Investigation and analysis of the incidence of recurrent oral ulcer in Xinjiang medical students,” Journal of Xinjiang Medical University, vol. 37, no. 1, pp. 117–120, 2014. View at Google Scholar
  21. L. P. Xie, J. X. Ye, Q. L. Cai et al., “Epidemiological survey of RAU in 631 Guangzhou medical students,” Guangdong Weiliang Yuansu Kexue, vol. 16, no. 6, pp. 12–15, 2009. View at Google Scholar
  22. L. Gavic, L. Cigic, D. Biocina Lukenda, V. Gruden, and J. S. Gruden Pokupec, “The role of anxiety, depression, and psychological stress on the clinical status of recurrent aphthous stomatitis and oral lichen planus,” Journal of Oral Pathology & Medicine, vol. 43, no. 6, pp. 410–417, 2014. View at Publisher · View at Google Scholar · View at Scopus
  23. M. K. Al-Omiri, J. Karasneh, M. M. Alhijawi, A. M. A. Zwiri, C. Scully, and E. Lynch, “Recurrent aphthous stomatitis (RAS): a preliminary within-subject study of quality of life, oral health impacts and personality profiles,” Journal of Oral Pathology & Medicine, vol. 44, no. 4, pp. 278–283, 2015. View at Publisher · View at Google Scholar · View at Scopus
  24. Y. Tian, M. Yang, F. Y. Yuan, B. J. Wang, and J. T. Wu, “Study on the change of pH value in oral cavity of patients with oral complication and its nursing,” Journal of Nurses Training, vol. 17, no. 3, pp. 171-172, 2002. View at Google Scholar
  25. D. L. Gambon, H. S. Brand, and A. V. Nieuw Amerongen, “Soft drink, software and softening of teeth - a case report of tooth wear in the mixed dentition due to a combination of dental erosion and attrition,” The Open Dentistry Journal, vol. 4, no. 1, pp. 198–200, 2010. View at Publisher · View at Google Scholar
  26. J. Zhou and Y. J. Zhu, “The experiment study of demineralization on the surface of the young permanent teeth enamel by carbonated beverage,” Journal of Clinical Stomatology, vol. 28, no. 3, pp. 182–184, 2012. View at Google Scholar
  27. B. T. Amaechi, S. M. Higham, and W. M. Edgar, “Influence of abrasion in clinical manifestation of human dental erosion,” Journal of Oral Rehabilitation, vol. 30, no. 4, pp. 407–413, 2003. View at Publisher · View at Google Scholar · View at Scopus
  28. M. Ogura, T. Yamamoto, M. Morita, and T. Watanabe, “A case-control study on food intake of patients with recurrent aphthous stomatitis,” Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, vol. 91, no. 1, pp. 45–49, 2001. View at Publisher · View at Google Scholar · View at Scopus
  29. R. F. Rong, “A comparison of the nutritional characteristics of several common nuts,” Journal of Beijing Union University (Natural Sciences), vol. 24, no. 1, pp. 12–16, 2010. View at Google Scholar
  30. J. Bao, Q. J. Wang, S. M. Li et al., “Case-control study of inducing factors in Shanghuo,” China Journal of Traditional Chinese Medicine and Pharmacy, vol. 30, no. 4, pp. 1013–1016, 2015. View at Google Scholar
  31. X. Baur and G. Fruhmann, “Allergic reactions, including asthma, to the pineapple protease bromelain following occupational exposure,” Clinical Allergy, vol. 9, no. 5, pp. 443–450, 1979. View at Publisher · View at Google Scholar · View at Scopus
  32. H. L. Zhao, “Prevent allergies on eating pineapples,” Home Medicine, vol. no. 7, p. 87, 2015. View at Google Scholar
  33. Y. Gu, R. C. Guo, H. M. Li, and H. Q. Chang, “The Research for change of the secretory capacity of salivary gland in RAU,” West China Journal of Stomatology, vol. 14, no. 4, pp. 272-273, 1996. View at Google Scholar
  34. S. M. Li, J. Bao, L. J. Mei, Q. J. Wang, Z. J. Xie, and Y. S. Fan, “On Shanghuo and diet,” Heilongjiang Journal of Traditional Chinese Medicine, vol. 44, no. 2, p. 6, 2015. View at Google Scholar
  35. R. V. Lalla, L. E. Choquette, R. S. Feinn et al., “Multivitamin therapy for recurrent aphthous stomatitis: a randomized, double-masked, placebo-controlled trial,” The Journal of the American Dental Association, vol. 143, no. 4, pp. 370–376, 2012. View at Publisher · View at Google Scholar · View at Scopus
  36. J. J. Lv, Y. G. Wang, and Q. Yang, “Analysis on composition rules of prescriptions in treating recurrent oral ulcer based on traditional Chinese medicine inheritance support,” Chinese Journal of Experimental Traditional Medical Formulae, vol. 22, no. 5, pp. 231–234, 2016. View at Google Scholar
  37. Y. F. He and K. Tian, “Sijunzi Tang plus Ganjiang treats recurrent aphthous stomatitis due to deficiency cold in the spleen and stomach,” World Health Digest, vol. 9, no. 6, pp. 171-172, 2012. View at Google Scholar