Zanthoxylum ailanthoides Suppresses Oleic Acid-Induced Lipid Accumulation through an Activation of LKB1/AMPK Pathway in HepG2 Cells

Figure 1

Effect of ZA-M and OA on HepG2 cell viability. (a and b) HepG2 cells were treated with various concentrations of ZA-M (10, 30, 50, and 100 μg/ml) and OA (100, 250, 500, and 1000 μM) for 24 h, respectively. Then, MTT assay was performed. (c) The cells were exposed to different concentrations of OA for 24 h, followed by Oil Red O staining to determine the lipid accumulation. (d) The cells were pretreated with indicated concentrations of ZA-M for 1 h, then exposed to 500 μM OA for 24 h. Cell viability was measured by MTT assay. The bar graphs show the mean ± SD of 3 independent experiments ( and compared with the DMSO control; , , and compared with the OA treated control).

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