Zanthoxylum ailanthoides Suppresses Oleic Acid-Induced Lipid Accumulation through an Activation of LKB1/AMPK Pathway in HepG2 Cells

Figure 2

Effect of ZA-M on OA-induced intracellular lipid accumulation in HepG2 cells. (a) The cells were pretreated with indicated concentrations of ZA-M for 1 h, followed by exposed to 500 μM OA for 24 h. Lipid accumulation was determined by using Oil Red O and BODIPY493/503 staining. Nuclei were counterstained with Hoechst 33342 dye. (b) Quantification of intracellular lipid accumulation. Total lipids stained with Oil Red O were extracted in absolute isopropanol, after which the absorbance of the solution was measured at 500 nm. (c) Quantitative analysis was performed by flow cytometry after BODIPY493/503 staining. (d) Quantification of TG contents by using commercial kit. The bar graphs show the mean ± SD of 3 independent experiments ( compared with the DMSO control; and compared with the OA treated control).

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