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(b)
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Figure 3: Effect of ZA-M on transcriptional/translational expression of lipogenic genes and sequential TG biosynthesis in HepG2 cells. (a) Western blot analysis of lipogenic protein expression level was performed after treatment of indicated concentrations of ZA-M (0, 50, 30, and 10 μg/ml) in HepG2 cells. (b) De novo TG biosynthesis. The cells were cotreated with various concentrations of ZA-M and [14C] glycerol for 6 h, and then TLC-based analysis of lipid intermediates was performed. Each [14C] TG band was quantified by using the Multi-Gauge V3.0 software (Fujifilm). The relative activity was calculated by setting the value from DMSO-treated cells to 100%. (c and d) Effect of ZA-M on OA-induced upregulation of transcriptional/translational expression of lipogenic genes was analyzed RT-PCR and western blot, respectively. The bar graphs show the mean ± SD of 3 independent experiments ( and compared with the DMSO control).