Antileishmanial and Immunomodulatory Effect of Babassu-Loaded PLGA Microparticles: A Useful Drug Target to Leishmania amazonensis Infection

Figure 2

Plasmon interaction of the aqueous extract of babassu mesocarp and of pentamidine with Leishmania amazonensis antigens (a), ultraviolet spectra (b), and FTIR spectra (c) of babassu-loaded microparticles (MMP) compared to free babassu mesocarp extract (Meso) and unloaded (control) microparticles (CMP). For analysis of the molecular interaction by surface plasmon resonance, aqueous babassu mesocarp, or pentamidine (100, 50, and 25 μg/mL) was bound to the ligand (Leishmania amazonensis crude extract, 150 μg/mL, pH 5.5) immobilized on a CM-5 sensor chip. The ultraviolet spectra (b) show absorption of the samples at a wavelength of 200 to 400 nm. Individual samples were tested in triplicate and the data refer to one of the samples. () p<0.05 compared to pentamidine. RU = resonance unit.

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