Research Article
Antileishmanial and Immunomodulatory Effect of Babassu-Loaded PLGA Microparticles: A Useful Drug Target to Leishmania amazonensis Infection
Figure 4
Production of hydrogen peroxide (a), TNF- (b), IL-6 (c), and IL-10 (d) by peritoneal macrophages incubated or not with microparticles. The results obtained for uninfected and untreated macrophage cultures (untreated) were compared to those of cultures treated for 24 h with babassu-loaded (MMP) or unloaded (CMP) microparticles. Spontaneous production of hydrogen peroxide (H2O2) or production stimulated with PMA (a) was evaluated using dihydrorhodamine as fluorescent probe. The concentration of TNF-α (b), IL- 6 (c), and IL-10 (d) was evaluated by ELISA. Results are the mean ± standard deviation of individual samples tested in triplicate. () p<0.05 compared to the untreated group; (#) p<0.05 compared to the CMP group.
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