Antileishmanial and Immunomodulatory Effect of Babassu-Loaded PLGA Microparticles: A Useful Drug Target to Leishmania amazonensis Infection

Figure 6

Production of cytokines and release of nitrite in peritoneal macrophage cultures infected with Leishmania amazonensis. Peritoneal macrophages infected with L. amazonensis were treated with 100 μg/mL babassu-loaded microparticles (MMP), unloaded microparticles (CMP, negative control), or pentamidine (Penta, positive control). These groups were compared to untreated infected macrophages (untreated). After 48 h, the concentrations of TNF-α (a), IL-6 (b), and IL-10 (c) were evaluated in the supernatants by ELISA and nitrite production (d) was measured with the Griess reagent. () p<0.05 compared to the untreated group; (#) p<0.05 compared to the CMP group.

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